Journal: BMC Biology

Article Title: Proteomics analysis of human mesenchymal stromal/stem cell sarcomagenesis model identifies ALDH1A3 and CD99 as potential targets in the transformation process

doi: 10.1186/s12915-025-02498-z

Figure Lengend Snippet: Myxoid liposarcoma tissues present low levels of CD99 expression. A Representative image of tissue microarray staining of CD99 in Ewing’s sarcoma, liposarcoma myxoid, osteosarcoma, and chondrosarcoma taken with Objective 10 ×. B Quantification of CD99 signal in cancer tissue microarray. Each dot represents one patient-derived sample, where the signal was normalized to Ewing’s sarcoma signal, and then regrouped by cancer type. N = 2 independent experiments were averaged. Ewing’s sarcoma is represented by a dotted line at 100% and serves as a positive control for CD99 abundance level as previously reported

Article Snippet: The tissue microarray (#NBP2-30,330) was purchased from Novus Biologicals (Toronto, ON, Canada).

Techniques: Expressing, Microarray, Staining, Derivative Assay, Positive Control

Journal: Molecular Therapy

Article Title: TALEN-edited allogeneic inducible dual CAR T cells enable effective targeting of solid tumors while mitigating off-tumor toxicity

doi: 10.1016/j.ymthe.2024.08.018

Figure Lengend Snippet: Tumor localized FAP + CAFs inhibit CAR T cell intra-tumoral infiltration and anti-tumor cytotoxicity (A) Schematic illustrating T cell infiltration assay in tumor spheroids plated with or without CAFs. (B) Graph depicting flow cytometry quantitation of T cells infiltrated per tumor spheroids with or without CAFs, represented as percentage of T cell input. Bars show means ± SD, n = 3; p values determined by Student t test (two-tailed, unpaired). ns, not significant, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001. (C) Schematic of TRAC KO ML CAR T cell cytotoxicity assay against tumor spheroids of TNBC cell line MDA-MB-231-Luc alone or co-cultured with TNBC-derived CAFs. (D) Bar graph representing percentage MDA-MB-231-Luc tumor cell survival post cytotoxicity assay outlined in (C), at Effector:Target ratio = 5:1. Bars show means ± SD, n = 3; p values determined by Student t test (two-tailed, unpaired). ns, not significant, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01. (E) Immunohistochemistry for detection of human FAP protein in tissue microarray of patient samples from different cancers. (F) Heatmap depicting percentage positive area stained for human FAP protein in immunohistochemical analysis of healthy human tissue microarray.

Article Snippet: Human tumor tissue and healthy tissue microarray slides were obtained from Novus Biologicals (NBP2-30234, NBP2-78082, NBP2-30232, NBP2-30233, NBP2-30189).

Techniques: Flow Cytometry, Quantitation Assay, Two Tailed Test, Cytotoxicity Assay, Cell Culture, Derivative Assay, Immunohistochemistry, Microarray, Staining, Immunohistochemical staining